Midori Green Advance

Article number: MG04

1 ml Midori Green Advance DNA/RNA DNA stain for in gel or poststaining

Midori Green Advance – the safe alternative to ethidium bromide

Midori Green Advance was developed to offer a non-carcinogenic and less mutagenic dye for detecting dsDNA, ssDNA and RNA in agarose gels. Besides these positive effects for your health Midori Green Advance DNA Stain shows a very high sensitivity even for small fragments as well as a superior signal to noise ratio. The performance and use of Midori Green Advance are in no way inferior to ethidium bromide (EtBr). The signal intensity is absolutely at least on a par with EtBr like the comparison of the following gels shows:

Performance comparison of Midori Green Advance and ethidium bromide
Ethidium bromide (left) in comparison with Midori Green Advance (right) on UV table

Data kindly provided by Mr. Schuster, Friedrich-Alexander-University Erlangen-Nürnberg, Germany.

Midori Green Advance vs. EtBr
Comparison of ethidium bromide and Midori Green Advance on a UV table

Comparison of sensitivity between ethidium bromide (left) and Midori Green Advance (right) using an UV transilluminator (invers projection).
Data kindly provided by Ms. Abel, Heinrich-Heine-University Düsseldorf; Germany.

Customer Feedback

“…we have tested Midori Green Advance and we are very excited about it. Please find attached to this email a typical picture….”

Dr. Olaf Voolstra,
Biosensor Research Group, Hohenheim University
Stuttgart

 

Precasting

  1. Prepare 100 ml of agarose gel solution (concentration from 0.8-3.0%) and heat until the solution is completely clear and no small floating particles are visible.
  2. Add 4-6 µl of Midori Green Advanced DNA Stain to the gel solution and mix it gently. Cool the gel to 50- 60ºC and cast the gel, into the gel tray. When the gel is solid, load the samples and perform electrophoresis.
  3. Detect the bands using a UV or LED illuminator. Yellow or green gelatin- or cellophane filters should be used for photography.

Poststaining

  1. Midori Green Advanced DNA Stain poststaining solution may be used 2-3 times.
  2. Staining solution that is going to be reused should be preferably stored at room temperature in the dark.
  3. For <0.5 cm thick agarose gel, 10-25 µl of the stain should be used per 100 ml of buffer.
  4. Optimal staining time (5 – 60 minutes) and the amount of the stain may depend on the thickness of the gel.

NOTICE: Usage is not recommended with SDS containing loading buffers because of band appearance caused by stain and SDS interaction!

D. Tacke, Georg-August-University Göttingen, plant pathology and plant protection

on 11/23/2017

Wir verwenden Midori Green Advance seit 2014 in unserer Abteilung und sind sehr zufrieden damit. Sowohl im täglichen Laboralltag als auch in Studentenpraktika liefert der Farbstoff sehr gute Ergebnisse.

Translated by Nippon Genetics:

We use Midori Green Advance in our department since 2014 and are very satisfied with it. In everyday laboratory practice as well as in practical course for students the stain delivers very good results.

D. Tacke, Georg-August-Universität Göttingen, Pflanzenpathologie und Pflanzenschutz

on 11/22/2017

Wir verwenden Midori Green Advance seit 2014 in unserer Abteilung und sind sehr zufrieden damit. Sowohl im täglichen Laboralltag als auch in Studentenpraktika liefert der Farbstoff sehr gute Ergebnisse.

Dr. Olaf Voolstra, Biosensor Research Group, Hohenheim University Stuttgart

on 10/24/2017

“…we have tested Midori Green Advance and we are very excited about it….”

Dr. Olaf Voolstra von der Universität Hohenheim, Fg. Biosensorik aus Stuttgart

on 10/24/2017

„…haben wir das Midori Green Advance getestet und sind begeistert!... "

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