FastGene Gradient PCR Cycler

Compact 96-well gradient PCR cycler for reliable routine PCR, optimization, and assay development

  • 96-well block for high sample throughput
  • Compatible with standard 0.2 mL PCR tubes, strips, and PCR plates
  • Very fast ramp rates of up to 5°C/s
  • Large 7-inch display with simple and intuitive software
  • Precise temperature control for reproducible results
  • Integrated gradient of up to 42°C, ideal for PCR optimization
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Cat. No. FG-TC03

Details

Flexible performance for everyday PCR workflows

The FastGene® Gradient PCR Cycler is a compact benchtop thermocycler for endpoint PCR with a 96-well block. The instrument operates quietly and features easy-to-learn, intuitive software.

The large 7-inch touchscreen is easy to use in any laboratory environment and supports comfortable day-to-day operation.

With intuitive buttons and a clearly structured interface, PCR programs and applications can be set up quickly and easily. This makes the system ideal for users of all experience levels.

The software is clearly organized and can be learned in a short time.

In addition, the FastGene® Gradient PCR Cycler offers an integrated gradient of up to 42°C, making it ideal for optimizing PCR protocols. Excellent heating and cooling rates, together with precise temperature control, ensure reliable and reproducible results.

FastGene Gradient PCR Cycler Gradient settings

Optimize PCR conditions efficiently

Primer annealing temperature strongly influences PCR specificity and product yield. The integrated gradient function allows multiple temperatures to be tested simultaneously across the 96-well block, helping identify suitable reaction conditions in a single run. With a gradient span of up to 42°C, new assays can be optimized faster while reducing repeated experiments and reagent use.

Gradient setting range 33-99°C
Maximum gradient span 42°C across the block
FastGene Gradient PCR Cycler | Block uniformity

Reliable conditions in every well

High temperature accuracy, precision, and block uniformity support reproducible PCR conditions across all 96 wells. This helps minimize variation between edge and center positions and contributes to consistent amplification results throughout the plate.

Temperature accuracy ±0.1°C
Temperature precision ±0.1°C
Temperature uniformity ±0.3°C at 55°C
Temperature setting range 0–105°C
FastGene Gradient PCR Cycler | Software

Straightforward setup

The 7-inch touchscreen and graphical user interface support straightforward protocol setup and daily operation. Up to 5,000 programs can be stored, while multi-user management and USB-based method transfer make the FG-TC03 suitable for shared laboratory environments.

Display 7-inch touchscreen
Program storage capacity Up to 5,000 programs
User management Up to 50 users
Internal storage 5 GB
Data import and export USB supported
Touchdown function Yes
FastGene Gradient PCR Cycler | Key applications

High performance across key PCR applications

From assay optimization to routine and multiplex PCR, the FG-TC03 supports reliable amplification across different experimental requirements. The agarose gel results demonstrate efficient annealing temperature optimization, consistent performance across all 96 wells, and simultaneous amplification of multiple target fragments.

Specifications

Parameter Specification
Product type Endpoint PCR thermocycler with gradient function
Sample capacity 96-well block
Sample volume range 5-100 µL
Maximum ramp rate 5°C/s
Average ramp rate ≥2°C/s
Temperature accuracy ±0.1°C
Temperature precision ±0.1°C
Temperature uniformity ±0.3°C at 55°C
Temperature setting range 0-105°C
Gradient setting range 30-99°C
Gradient range 42°C across the block
Touchdown function Yes
Lid temperature 30-105°C
Display 7-inch touchscreen
User interface Graphical touchscreen interface
Program storage capacity Up to 5000 programs
Internal storage 5 GB
RAM 1 GB
User management Up to 50 users
Data import/export USB supported
Software updates Via USB
Dimensions (width x depth x height) 288 mm x 427 mm × 227 mm
Weight 9.7 kg
Intended use Research use only

 

Compatibility

The FastGene® Gradient PCR Cycler can be combined with compatible FastGene® tubes, strips, and plates for a reliable and complete endpoint PCR setup.

Category
Product Description
Cat. No.
PCR plastics – Individual tubes PCR Tubes 0.2 mL with domed caps, 1000 pcs FG-021D
PCR Tubes 0.2 mL with flat caps, 1000 pcs FG-021DF
PCR Tubes 0.1 mL with flat caps, 1000 pcs FG-011F
PCR plastics – 8-well strips 8-well PCR Tube Strips 0.2 mL with Flat Cap Strips, 120 pcs FG-016FC
8-well PCR Tube Strips 0.2 mL without caps, 120 pcs FG-028
Flat Cap Strips for 0.2 mL strips, 120 pcs FG-008FC
8-well PCR Strips 0.2 mL with single flat caps, 120 pcs, DE FG-088WFDE
PCR plastics – 96-well plates FastGene 96-well PCR Plate 0.2 mL, nonskirted, 25 pcs FG-170225
FastGene 96-well PCR Plate 0.2 mL, semi-skirted, 50 pcs FG-190250
FastGene 96-well PCR ABI Plate 0.2 mL, semi-skirted, 50 pcs FG-200250

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More information

Manual

Manual | FastGene Gradient PCR Cycler | FG-TC03

Quick guide

Quick guide | System setup | FastGene Gradient PCR Cycler | FG-TC03

Brochure

Brochure | FastGene Gradient PCR Cycler | FG-TC03

Product inquiry

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