FastGene HiFi HS 2x Master Mix

High fidelity polymerase

  • Reliable high fidelity polymerase
  • Superior performance under difficult conditions, e.g., AT or GC-rich sequences and long templates
  • Perfect for mulitplex-PCRs and applications where high accuracy is needed (e.g., cloning, site-directed mutagenesis, sequencing)
  • Ready-to-use cocktail including all components neccessary for a wide range of targets and fragment sizes
  • 3’-5’ exonuclease and proof-reading activity
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Cat. No. LS36 100 rxns

Details

High Fidelity is key

The high fidelity of the FastGene® HiFi Polymerase is based on the improved 3`-5` exonuclease activity, which significantly reduces the error rate of the enzyme and makes it around 100 times more accurate than a Taq DNA polymerase. This is ideal for applications where high fidelity is essential, such as sequencing, cloning and site-directed mutagenesis.

Save time with the Master Mix

The FastGene® HiFi Polymerase is provided in a 2x Master Mix, which significantly speeds up the preparation of a PCR. It contains an advanced buffering system with dNTPs, Mg2+, reaction enhancers and the polymerase enzyme.

Product applications

The FastGene® HiFi HS 2x Master Mix (LS36) is ideally suited for:

  • Accurate Cloning
  • Sequencing
  • Site-directed mutagenesis
  • High fidelity PCR
  • Long range PCR
  • Any standard PCR application for which a hot start formulation of a high-quality thermostable DNA polymerase is require
Challenging PCR? Perfect for FastGene® HiFi Polymerase!

The FastGene® HiFi Polymerase is the high-fidelity enzyme for precise PCR amplifications. The enzyme was engineered in a way that it can amplify particularly long templates (up to 17.5 kb) with a high sequence accuracy. Furthermore, it shows a significant improvement in extension times (10-30 sec per kb), while generating high yields, even with difficult templates.

Amplify long fragments

Figure 1: The FastGene® HiFi Polymerase is capable of amplifying a long range of fragments, even up to 17500 bp. (The image was generated with MIDORIGreen Xtra safe DNA dye and FastGene® FAS-DIGI PRO gel documentation system.)

Deal with tough templates

Figure 2: Templates with varying GC-content, ranging from ~30 % – 80 % can be successfully amplified with the FastGene® HiFi Polymerase. (The image was generated with MIDORIGreen Xtra safe DNA dye and FastGene® FAS-DIGI PRO gel documentation system.)

Perform easy multiplexing

Figure 3: 6 phage DNA fragments, 4 mouse DNA fragments and a combination of both were successfully amplified with the FastGene® HiFi Polymerase in a multiplex PCR. (The image was generated with MIDORIGreen Xtra safe DNA dye and FastGene® FAS-DIGI PRO gel documentation system.)

Free sample

Convince yourself and ask for a free sample!

Downloads

Manual

Manual – FastGene® HiFi HS 2x Master Mix (LS36)

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