Bambanker DMSO Free

Serum-free cryopreservation media for most sensitive cells

  • DMSO-free – for the most sensitive cells
  • Enables long term cell storage at -80°C or -196°C
  • Improved cell recovery and viability after thawing
  • Ready-to-use freezing media – no programmed or sequential freezing required
  • Serum-free – no risk of contamination
  • GMP manufactured
  • Usable for all known cell lines
  • Long shelf life


Price on request

Cat. No. BBF01


No more DMSO – for the most sensitive cells

DMSO is added to most freezing reagents to help avoid formation of ice crystals, which of course harm the cells. However, DMSO is cytotoxic and can reduce the survival rate of certain sensitive cell lines. Bambanker DMSO Free is made without DMSO. Instead, it uses a unique formulation to avoid the formation of ice crystals. This makes Bambanker DMSO Free especially suitable for cell lines that proved to be sensitive to DMSO under long-term storage conditions.


There a four different versions of Bambanker™.

  • All Bambanker™ types are free of fetal bovine serum (FBS) and have a defined and constant composition.
  • They also do not contain antibiotic or antifungal ingredients.

The four different types of Bambanker™ are recommended depending on your specific needs:

BB03-Bambanker- 20mL Bambanker™ (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 120 mL)
Bambanker™ Direct (for hybridoma cells, package unit: 20 mL)
Bambanker™ hRM (for ES and iPS cells, package units: 20 mL, 120 mL)


GMP quality

GMP quality

All products of the Bambanker series are manufactured in facilities compliant with current good manufacturing practices (GMP).

The products are subject to a solid quality management system that ensures the consistency, reliability and high quality you can rely on.


Manufacturer’s notification for Bambanker as GMP-manufactured product (pdf file)

Cell lines & References

Cell lines

The following cell lines have successfully been frozen with Bambanker DMSO-free:

  1. Jurkat (Floating cells)
  2. PC12  (Adrenal Pheochromocytoma, Rat) (Adhesive cells)
  3. HepG2  (Adhesive cells)
  4. Hela S3  (Epithelioid Carcinoma, Cervix, Human)(Adhesive cells)
  5. HEK293T  (Transformed Primary Embryonal Kidney, Human)(Adhesive cells)
  6. OKT4  (Floating cells)
  7. Vero P4 (Kidney, African Green Monkey(Adhesive cells)
  8. MDCK (Kidney, Canine)(Adhesive cells)
  9. NIH3T3  (Embryonic Fibroblast, Mouse)(Adhesive cells)
Customer references

I consider Bambanker a very useful worktool for the following reasons:
1) replacement of a toxic substance (DMSO)
2) saving of 4 hours of time required for freezing in nitrogen / -80° C foreseen with the use of DMSO
3) cellular vitality guaranteed after the defrosting of all the linesthat I manage

Dott.ssa Allessandra Leone, Department of Virology and Cell Culture, IZSAM  “G.Caporale” Teramo

Literature reference

Effect of DMSO on cell differentitation:

Jiang, C. et al. Down-regulation of TRRAP-dependent hTERT and TRRAP-independent CAD activation by Myc/Max contributes to the differentiation of HL60 cells after exposure to DMSO International Immunopharmacology, 6 (7), 1204-1213 (2006)



Manual | Bambanker DMSO-Free (BBF0#)


Flyer Bambanker Freezing Media


MSDS | Bambanker DMSO Free (BBF0#)


3 reviews for Bambanker DMSO Free

  1. desiree.argiriu

    I needed a DMSO-free Medium for my very sinsitive Mastcells and it worked very nice! The medium helped that after freezing and thawing the cells grow faster and have a higher viability. Nice product!

    Verified User

  2. Dr. Céline Laschet, BCiPharma, Belgium

    We observed that there were more living cells after thawing (4.5 times more cells after 3 days of thawing) but not yet enough cells to pass into a T75. It took 7 days of thawing for the passage in a T75 vs 10 days for our classic freezing condition.

    Verified User

  3. Mady, University Tübingen, Germany

    We had a major liquid Nitrogen tank issue. We looked for an alternative. We requested a free sample to try.
    Bambanker was sooo easy to use and very good on sensitive cells (no toxicity see), which can also be stored at -80. No more DMSO/FBS!!
    I highly recommend it (10 years+ (primary& cell line) cell culture experience!)

    Verified User

Only logged in customers may leave a review.