A new hope for the cryopreservation of ES and iPS cells
Primate embryonic stem cells and induced-pluripotent stem cells are extremely sensitive to cryopreservation which presents many difficulties when compared to murine or other cells. At present, the vitrification method is considered adequate for primate ES/iPS cells although the slow-freezing method using DMSO has been popular for a wide variety of cell lines. Vitrification is the rapid cooling of the freezing media to a glass-like crystalline state. The vitrification method requires impeccable timing, a high level of skill, and still can yield poor results. In addition, this method shows sensitivity to dry ice transportation. To address these problems, the new freezing medium BambankerTM HRM has been developed.
What makes BambankerTM HRM so special?
The Cell freezing media BambankerTM HRM contains no bovine serum albumin, which can sometimes lead to cell differentiation. Furthermore, there are no animal-derived material which improve storage and survivability of primate ES/iPS cells while greatly simplifying the protocol.
Cell freezing without Serum!
All BambankerTM products are produced with no serum. Cryopreservation media which contain sera have the disadvantage of fluctuations in recovery rates and undefined composition. Reproducibility of experiments with cells which were frozen in a serum-containing-medium, could be affected by lot-to-lot variation of the serum since the composition and concentration of proteins and other biological molecules varies with each batch of serum. This may result in issues when thawing and using cells from such serum-containing media. Breathe easy, as every ingredient of BambankerTM is precisely defined so that you can be confident that cells stored at different times will all behave and recover similarly.
There a four different version of BambankerTM:
- BambankerTM (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 120 mL)
- BambankerTM Direct (for hybridoma cells, package unit: 20 mL)
- BambankerTM DMSO Free (for high sensitivity cells, package unit: 20 mL)
- BambankerTM hRM (for ES and iPS cells, package units: 20 mL, 120 mL)