Details
Bambanker™ Direct for hybridomas
There are certain cell types, such as hybridomas, which exhibit increased sensitivity to “over” manipulations. These cells often exhibit higher death rates or unwanted differentiation after long-term storage and freeze thaw cycling. It is for these types of cells that Bambanker™ Direct was created – to provide tight environmental control. By eliminating the centrifugation step, cells can now be frozen for long-term storage immediately upon addition of the novel cryoprotectant. Just add Bambanker™ Direct, one-toone, with your cells in media and directly place in freezer. And like with the original Bambanker™, the Direct version has no serum, so it is great for cells which animal-derived serum may be an issue.
Procedure for the cryopreservation of cells
Flexible
There a four different versions of Bambanker™.
- All Bambanker™ types are free of fetal bovine serum (FBS) and have a defined and constant composition.
- They also do not contain antibiotic or antifungal ingredients.
The four different types of Bambanker™ are recommended depending on your specific needs:
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Bambanker™ (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 120 mL) |
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Bambanker™ Direct (for hybridoma cells, package unit: 20 mL) |
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Bambanker™ hRM (for ES and iPS cells, package units: 20 mL, 120 mL) |
Researcher from Japan –
I am using Bambanker Direct for frozen hybridomas when making monoclonal antibodies.
After screening, the culture of positive cells was scaled from 96 wells to 24 wells. Pipette 250 μl of cells in the growth phase from the centre where the 24 well cells are dense, mix it with an equal amount of Bambanker Direct, and freeze. Freezing can be done quite easily without the need to centrifuge the cells and so on. I think that it will be powerful for freezing floating cells. Bambanker is used for adhesion cells and Bambanker Direct is used for floating cells.
Verified User
Mady , Tübingen University –
We had a major liquid Nitrogen tank issue. We looked for an alternative. We requested a free sample to try.
Bambanker was sooo easy to use and very good on sensitive cells (no toxicity see), which can also be stored at -80. No more DMSO/FBS!!
I highly recommend it (10 years+ (primary& cell line) cell culture experience!)
Verified User