BambankerTM Direct for hybridomas
There are certain cell types, such as hybridomas, which exhibit increased sensitivity to “over” manipulations. These cells often exhibit higher death rates or unwanted differentiation after long-term storage and freeze thaw cycling. It is for these types of cells that BambankerTM Direct was created – to provide tight environmental control. By eliminating the centrifugation step, cells can now be frozen for long-term storage immediately upon addition of the novel cryoprotectant. Just add BambankerTM Direct, one-toone, with your cells in media and directly place in freezer. And like with the original BambankerTM, the Direct version has no serum, so it is great for cells which animal-derived serum may be an issue.
Procedure for the cryopreservation of cells
There a four different version of BambankerTM:
- BambankerTM (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 120 mL)
- BambankerTM Direct (for hybridoma cells, package unit: 20 mL)
- BambankerTM DMSO Free (for high sensitivity cells, package unit: 20 mL)
- BambankerTM hRM (for ES and iPS cells, package units: 20 mL, 120 mL)
Researcher from Japan –
I am using Bambanker Direct for frozen hybridomas when making monoclonal antibodies.
After screening, the culture of positive cells was scaled from 96 wells to 24 wells. Pipette 250 μl of cells in the growth phase from the centre where the 24 well cells are dense, mix it with an equal amount of Bambanker Direct, and freeze. Freezing can be done quite easily without the need to centrifuge the cells and so on. I think that it will be powerful for freezing floating cells. Bambanker is used for adhesion cells and Bambanker Direct is used for floating cells.
Mady , Tübingen University –
We had a major liquid Nitrogen tank issue. We looked for an alternative. We requested a free sample to try.
Bambanker was sooo easy to use and very good on sensitive cells (no toxicity see), which can also be stored at -80. No more DMSO/FBS!!
I highly recommend it (10 years+ (primary& cell line) cell culture experience!)