Bambanker Direct

Cryopreservation media

Serum free freezing media for long storage of cells

  • Enables long term cell storage at -80°C or -196°C
  • Perfecty suited for hybridoma cells and HTP-applications
  • Bambanker Direct can directly added to the cells – No washing and no centrifugation necessary
  • Improved cell recovery and viability after thawing
  • Ready-to-use freezing media – no programmed or sequential freezing required
  • Serum-free – no risk of contamination
  • Long shelf life

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Cat. No. BBD01

Details

BambankerTM Direct for hybridomas

There are certain cell types, such as hybridomas, which exhibit increased sensitivity to “over” manipulations. These cells often exhibit higher death rates or unwanted differentiation after long-term storage and freeze thaw cycling. It is for these types of cells that BambankerTM Direct was created – to provide tight environmental control. By eliminating the centrifugation step, cells can now be frozen for long-term storage immediately upon addition of the novel cryoprotectant. Just add BambankerTM Direct, one-toone, with your cells in media and directly place in freezer. And like with the original BambankerTM, the Direct version has no serum, so it is great for cells which animal-derived serum may be an issue.

Procedure for the cryopreservation of cells

Vergleich des Protokolls für Bambanker und Bambanker Direct

Flexible

There a four different version of BambankerTM:

  • BambankerTM (standard for all cell lines, package units: 20 mL, 5 x 20 mL, 120 mL)

BB03-Bambanker- 20mL

Cell Lines

Cell lines succesfully stabilized by BambankerTM Direct:
  • C2C12 (mouse skeletal muscle cell line)
  • Daudi (human B cell line)
  • HEK293; HEK293T (human embryonic kidney cell line)
  • HeLa/HeLa S3 (human cervical cancer cell line)
  • HepG2 (human liver cancer cell line)
  • Jurkat (human leukemia T cell line)
  • K562 (human chronic myelogenous leukemia cell line)
  • KATO (human gastric epithelial cell line)
  • MDCK (canine kidney tubular epithelial cell line)
  • NIH3T3 (mouse embryonic skin cell line)
  • OKT4 (mouse hybridoma)
  • OP9 (mouse myeloid stromal cells)
  • PC12 (rat adrenal medulla cell line)
  • SNL (mouse embryonic fibroblasts)
  • ES (mouse embryonic stem cells)
  • MEF (mouse embryonic fibroblasts)
  • Vero (African green monkey kidney cell line)
  • OP9 (mouse myeloma stroma cells)
  • P3U1 (mouse myeloma cell line)

and many more…

Documents

BBD0_Bambanker-Direct_Manual
Bambanker Stem-cells flyer
Cell biology Products

2 reviews for Bambanker Direct

  1. Researcher from Japan

    I am using Bambanker Direct for frozen hybridomas when making monoclonal antibodies.
    After screening, the culture of positive cells was scaled from 96 wells to 24 wells. Pipette 250 μl of cells in the growth phase from the centre where the 24 well cells are dense, mix it with an equal amount of Bambanker Direct, and freeze. Freezing can be done quite easily without the need to centrifuge the cells and so on. I think that it will be powerful for freezing floating cells. Bambanker is used for adhesion cells and Bambanker Direct is used for floating cells.

  2. Mady , Tübingen University

    We had a major liquid Nitrogen tank issue. We looked for an alternative. We requested a free sample to try.
    Bambanker was sooo easy to use and very good on sensitive cells (no toxicity see), which can also be stored at -80. No more DMSO/FBS!!
    I highly recommend it (10 years+ (primary& cell line) cell culture experience!)

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