Real-time fluorescent quantitative PCR system | NEW: 6 channel system
Fast detection − Probe & dye-based assays
6 different colour channels – perfect for multiplex qPCR(view channels)
Also available with 4+1 channels: 4 different colour channels + 1 additional second FAM/SYBR channel for fast dual mode melt curve analysis (FastGene® qFYR, FG-QPTC01)
Standard plate format − 96-well block with gradient function
Highest sensitivity
Outstanding homogeneity across all wells of a 96-well plate
The FastGene® qFYR is a highest precision instrument for performing quantitative polymerase chain reaction (qPCR) experiments. qPCR is a well-established method for the sensitive detection and quantification of nucleic acids. During a measurement, the target DNA or RNA sequence is amplified, while a cycle-dependent increase of a fluorescent signal is detected in real-time.
The device for multiple applications
The FastGene® qFYR was developed to meet highest laboratory standards and deliver reliable performances for various real-time qPCR applications:
Gene expression analysis
Absolute and relative quantification
Endpoint qualitative analysis
Genotyping
Gene mutation analysis
Pathogen detection
GMO detection
Protein stability screening
miRNA analysis
Melting curve analysis
Innovative Technology
High sensitivity optical design
The FastGene® qFYR has a unique, patented optical detection system. It combines a high quality PMT (photomultiplier tube) with a Fresnel lens that has a short focal length. The resulting short distance from detector to sample reduces signal loss and cross-talk between samples, and generally improves signal sensitivity.
Superior thermal cycler performance
The FastGene® qFYR delivers excellent temperature control, temperature precision (± 0.2 °C) and uniformity over the entire 96-well plate. The unique hollowed out thermal block design reduces its overall weight and ensures rapid heating and cooling rates (up to 6 °C per second) for fast qPCR protocols. The system also uses state-of-the-art Peltier components for highest reaction quality and performance stability, providing reliable and consistent qPCR results.
Fast measurement with high performance LED channels
Due to the small distance between the scanning head and the plate, the FastGene® qFYR has an outstanding level of sensitivity and virtually no cross talk between the individual wells.
4 or 6 different colour channels are installed in the scanning head to be able to excite all commonly used qPCR dyes in a single plate scan (8 sec).
The FastGene® qFYR is available in 2 different versions, with:
The FastGene® qFYR (FG-QPTC01) has 4+1 channels: 4 different colour channels and 1 second FAM channel.
The dual FAM/SYBR channel allows melt curve and high resolution melt experiments to be performed in fast dual mode, halving the measuring time in this channel.
Channel 1: FAM/SYBR Green etc.
Channel 2: VIC/JOE/HEX/TET etc.
Channel 3: ROX/Texas Red etc.
Channel 4: Cy5 etc.
Channel 5: FAM/SYBR Green etc.
II) FastGene® qFYR Plus (FG-QPTC02)
The FastGene® qFYR Plus (FG-QPTC02) has 6 different colour channels.
Channel 1: FAM/SYBR Green etc.
Channel 2: VIC/JOE/HEX/TET etc.
Channel 3: ROX/Texas Red etc.
Channel 4: Cy5 etc.
Channel 5: Cy5.5/Quasar 705/Alexa Fluor 680
Channel 6: ATTO 42
Software | Video tutorials
The powerful user software has it all
The analysis software (FastGene® qFYR Analysis Studio) impresses with its particularly simple and operation. The menu is clearly structured and intuitively arranged. It responds to the needs of the user for different experimental setups, and personalized settings can be easily adjusted. Integrated analysis algorithms allow many steps, such as baseline subtraction or Cq value threshold calculation, to be performed automatically.
Absolute and relative quantification of nucleic acids can also be automated. The software allows you to save predefined analysis settings for auto-exporting run data into a format of choice, including Excel, PDF, txt export format.
Clearly structured and user-friendly
Intuitive navigation
Customizable settings
Automatic analysis with integrated algorithms
Modules for multiple applications
Modern UI design
Video tutorial – Complete webinar | 25:22 min
See how easy it is to (1) install the software, (2) set up the experiments and (3) analyse the data.
Um das YouTube-Video zu sehen, müssen Sie Cookies akzeptieren.
Short video: How to set up your Real-Time PCR experiment in under 3 minutes | 2:51 min
Um das YouTube-Video zu sehen, müssen Sie Cookies akzeptieren.
Short video: How to analyse and export your real-time data in under 2 minutes | 1:29 min
Um das YouTube-Video zu sehen, müssen Sie Cookies akzeptieren.
Application data
Generate high quality data
The combination of innovative optics and a high-precision thermal block ensure ideal amplification conditions. This makes the FastGene® qFYR ideal for various quantitative real-time PCR applications.
Outstanding homogeneity
FastGene® qFYR shows great homogeneity across all wells of a 96-well plate.
Figure 1: Amplification of 1 ng plasmid DNA in all 96 wells of a PCR plate. The mean Cq value at a cycle number of 13.89 +/- 0.055 was determined automatically, illustrating highly homogenous amplification results obtained by the FastGene® qFYR.
Powerful multiplexing
FastGene® qFYR Plus can discriminate up to 6 different targets in a single reaction well, helping you save time, conserve reagents, and reduce samples usage without sacrificing precision.
Figure 2: Multiplex amplification of six mammalian housekeeping genes (GAPDH, B2M, ACTG1, PGK1, HPRT1, and ACTB ). Each gene was detected using a different fluorescent probe: FAM – GAPDH, HEX – B2M, TexasRed – ACTG1, Cy5.5 – PGK1, ATTO425 – HPRT1, Cy5 – ACTB.
Accurate quantification
The broad dynamic range of the FastGene® qFYR ensures a reliable and accurate quantification.
Figure 3: The amplification plot shows the log of change in normalized reporter fluorescence against the cycle number. The amplification took place from plasmid DNA with AMP specific primers in a 10-fold dilution series ranging from 1 ng to 1×10-6 ng plasmid DNA. The generated standard curve shows 100 % efficiency.
1.3-fold target discrimination
With the FastGene® qFYR, smaller concentration differences can be distinguished with high accuracy, underlining the high sensitivity of the device.
Figure 4: The amplification of plasmid DNA was carried out using AMP-specific primers with a 2-fold dilution series starting at 0.1 ng with additional dilutions of 1:1.3, 1:1.4, 1:1.5 and 1:1.6. Concentration differences can be detected up to 1:1.3-fold dilution.
Melt curve analysis
The Precision Melt Analysis Tool is a powerful method for probe-based allelic discrimination. This function is already included in the FastGene® qFYR analysis software and does not need to purchased additionally.
Figure 5: The difference plot of the high-resolution melting curve allows the discrimination between SNPs extracted from blood samples for brown eyes and two variants of blue eyes.
Gradient qPCR for optimal annealing
The thermal gradient function of the FastGene® qFYR can be used to determine the optimal annealing temperature of a specific target.
Figure 6: Plasmid amplification with IC green dye was carried out in a 10-fold dilution series from 1 ng to 1×10-6 ng. This example of a thermal gradient experiment with 55 °C and 68 °C shows 55 °C to be the optimal annealing temperature with a 100% efficiency.
Dimensions: 35 cm x 52 cm x 37 cm (length x wide x height)
Weight: 25 kg
Dimensions: 35 cm x 52 cm x 37 cm (length x wide x height)
Weight: 25 kg
Environmental conditions
Temperature: 10–30 °C
Humidity: ≤85 % RH no condensation
Atmosheric pressure: 85.0 kPa ~ 106.0 kPa
Altitude: < 2000 m
Temperature: 10–30 °C
Humidity: ≤85 % RH no condensation
Atmosheric pressure: 85.0 kPa ~ 106.0 kPa
Altitude: < 2000 m
Power supply specification
Power supply voltage: AC 100–240 V
Frequency: 50 Hz
Power: 750 W
Power supply voltage: AC 100–240 V
Frequency: 50 Hz
Power: 750 W
Data communication interface
USB ports
USB ports
Compatibility & Consumables
Get the right consumables for FastGene® qFYR
The FastGene® qFYR is compatible with low-profile (0.1 mL) PCR tubes/8-well PCR tube strips with transparent, flat tops as well as non-skirted or half-skirted low profile 96 well PCR reaction plates (See product category: PCR Plastic (link)):
Single tubes: Low-profile (0.1 mL) clear/white PCR thin-walled single tube, flat-topped transparent optical tube cover, e.g.:
FastGene® 96-well Fast PCR Plate 0.1 mL (FG-03890-50)
! It is not compatibel with high-profile (0.2 mL) PCR reaction tube and convex tube covers !
Info: If the sample tubes are just a few, use 8-well tubes for support on both sides, or use empty single tubes in the four corners to prevent uneven force on the plate slot.
F1: FAM/SYBR Green
F2: VIC/HEX/TET/JOE, JUN
F3: ROX/Texas Red, Mustang Purple
F4: Cy5/LIZ
F5: Cy5.5/Quasar 705/Alexa Fluor 680 (FastGene® qFYR Plus only)
F6: ATTO 425 (FastGene® qFYR Plus only)
Computer configuration requirements
The computer installed with the FastGene® qFYR Analysis Studio must have the following configuration: Processor: Intel or AMD dual-core CPU,2.8GHz Memory: 4 GB Hard disk: 500 GB Network card: 10 M/100M adaptive network card (optional) Display resolution: 1440×900 or higher Operating system: Windows 10 or above & Office Word/Excel 2007 or above.
Reviews
There are no reviews yet.