Highest precision instrument
The FastGene® qFYR is a highest precision instrument for performing quantitative polymerase chain reaction (qPCR) experiments. qPCR is a well-established method for the sensitive detection and quantification of nucleic acids. During a measurement, the target DNA or RNA sequence is amplified, while a cycle-dependent increase of a fluorescent signal is detected in real-time.
The device for multiple applications
The FastGene® qFYR was developed to meet highest laboratory standards and deliver reliable performances for various real-time qPCR applications:
- Gene expression analysis
- Absolute and relative quantification
- Endpoint qualitative analysis
- Gene mutation analysis
- Pathogen detection
- GMO detection
- Protein stability screening
- miRNA analysis
- Melting curve analysis
High sensitivity optical design
The FastGene® qFYR has a unique, patented optical detection system. It combines a high quality PMT (photomultiplier tube) with a Fresnel lens that has a short focal length. The resulting short distance from detector to sample reduces signal loss and cross-talk between samples, and generally improves signal sensitivity.
Superior thermal cycler
The FastGene® qFYR delivers excellent temperature control, temperature precision (± 0.2 °C) and uniformity over the entire 96-well plate. The unique hollowed out thermal block design reduces its overall weight and ensures rapid heating and cooling rates (up to 6 °C per second) for fast qPCR protocols. The system also uses state-of-the-art Peltier components for highest reaction quality and performance stability, providing reliable and consistent qPCR results.
Fast scanning mode − 4+1 channels
4 different fluorescence channels and a double FAM/SYBR channel enabling fast measurements.
Due to the small distance between the scanning head and the plate, the FastGene® qFYR has an outstanding level of sensitivity and virtually no cross talk between the individual wells.
Four different colour channels are installed in the scanning head to be able to excite all commonly used qPCR dyes in a single plate scan (8 sec).
The double FAM/SYBR channel allows to perform melt curve and high resolution melt experiments in fast dual mode, which halves the measuring time in this channel.
Channel 1: FAM/SYBR Green etc.
Channel 2: VIC/JOE/HEX/TET etc.
Channel 3: ROX/Texas Red etc.
Channel 4: Cy5 etc.
Channel 5: FAM/SYBR Green etc.
Software | Video tutorials
The powerful user software has it all
The analysis software (FastGene® qFYR Analysis Studio) impresses with its particularly simple and operation. The menu is clearly structured and intuitively arranged. It responds to the needs of the user for different experimental setups, and personalized settings can be easily adjusted. Integrated analysis algorithms allow many steps, such as baseline subtraction or Cq value threshold calculation, to be performed automatically.
Absolute and relative quantification of nucleic acids can also be automated. The software allows you to save predefined analysis settings for auto-exporting run data into a format of choice, including Excel, PDF, txt export format.
- Clearly structured and user-friendly
- Intuitive navigation
- Customizable settings
- Automatic analysis with integrated algorithms
- Modules for multiple applications
- Modern UI design
Video tutorial – Complete webinar | 25:22 min
See how easy it is to (1) install the software, (2) set up the experiments and (3) analyse the data.
Short video: How to set up your Real-Time PCR experiment in under 3 minutes | 2:51 min
Short video: How to analyse and export your real-time data in under 2 minutes | 1:29 min
Generate high quality data
The combination of innovative optics and a high-precision thermal block ensure ideal amplification conditions. This makes the FastGene® qFYR ideal for various quantitative real-time PCR applications.
FastGene® qFYR shows great homogeneity across all wells of a 96-well plate.
Figure 1: Amplification of 1 ng plasmid DNA in all 96 wells of a PCR plate. The mean Cq value at a cycle number of 13.89 +/- 0.055 was determined automatically, illustrating highly homogenous amplification results obtained by the FastGene® qFYR.
FastGene® qFYR can discriminate up to five different targets in a single reaction well.
Figure 2: Multiplex amplification of three gene targets (Actb, Gapdh and B2m) that was carried out from 100 ng to 0.1 ng RNA in a OneStep qPCR with the FastGene® Probe OneStep Mix (LS47).
The broad dynamic range of the FastGene® qFYR ensures a reliable and accurate quantification.
Figure 3: The amplification plot shows the log of change in normalized reporter fluorescence against the cycle number. The amplification took place from plasmid DNA with AMP specific primers in a 10-fold dilution series ranging from 1 ng to 1×10-6 ng plasmid DNA. The generated standard curve shows 100 % efficiency.
1.3-fold target discrimination
With the FastGene® qFYR, smaller concentration differences can be distinguished with high accuracy, underlining the high sensitivity of the device.
Figure 4: The amplification of plasmid DNA was carried out using AMP-specific primers with a 2-fold dilution series starting at 0.1 ng with additional dilutions of 1:1.3, 1:1.4, 1:1.5 and 1:1.6. Concentration differences can be detected up to 1:1.3-fold dilution.
Melt curve analysis
The Precision Melt Analysis Tool is a powerful method for probe-based allelic discrimination. This function is already included in the FastGene® qFYR analysis software and does not need to purchased additionally.
Figure 5: The difference plot of the high-resolution melting curve allows the discrimination between SNPs extracted from blood samples for brown eyes and two variants of blue eyes.
Gradient qPCR for optimal annealing
The thermal gradient function of the FastGene® qFYR can be used to determine the optimal annealing temperature of a specific target.
Figure 6: Plasmid amplification with IC green dye was carried out in a 10-fold dilution series from 1 ng to 1×10-6 ng. This example of a thermal gradient experiment with 55 °C and 68 °C shows 55 °C to be the optimal annealing temperature with a 100% efficiency.
Compatibility & Consumables
Get the right consumables for FastGene® qFYR
The FastGene® qFYR is compatible with low-profile (0.1 mL) PCR tubes/8-well PCR tube strips with transparent, flat tops as well as non-skirted or half-skirted low profile 96 well PCR reaction plates (See product category: PCR Plastic (link)):
- Single tubes: Low-profile (0.1 mL) clear/white PCR thin-walled single tube, flat-topped transparent optical tube cover, e.g.:
- 8-well strips: Low-profile (0.1 mL) clear/white PCR thin-walled 8-tube, flat-top transparent optical tube cover, e.g.:
- 8-well PCR Tube Strips 0.1 mL with flat cap strips (FG-017FC)
- 8-well PCR Tube Strips 0.1 mL with flat caps (FG-018WF)
- 8-well White PCR Tube Strips 0.1 mL + optical Flat Cap Strips (FG-019FC)
- 96-well plates: Low-profile (0.1 mL) clear/white PCR thin-walled 96- well plate with no hemline or hemline, e.g.:
- FastGene® White 96-well PCR Plate 0.1 mL, semi-skirted (FG-2102)
- FastGene® 96-well PCR Plate 0.1 mL, non-skirted (FG-170350)
- FastGene® 96-well Fast PCR Plate 0.1 mL (FG-03890-50)
! It is not compatibel with high-profile (0.2 mL) PCR reaction tube and convex tube covers !
Info: If the sample tubes are just a few, use 8-well tubes for support on both sides, or use empty single tubes in the four corners to prevent uneven force on the plate slot.
Our FastGene® IC Green and Probe Mixes are the ideal reagents for the FastGene® qFYR.
Find the right dye mix for your application: PCR reagents and enzymes – Quantitative PCR (link).
F1: FAM/SYBR Green
F2: VIC/HEX/TET/JOE, JUN
F3: ROX/Texas Red, Mustang Purple
Computer configuration requirements
The computer installed with the FastGene® qFYR Analysis Studio must have the following configuration:
Processor: Intel or AMD dual-core CPU,2.8GHz
Memory: 4 GB
Hard disk: 500 GB
Network card: 10 M/100M adaptive network card (optional)
Display resolution: 1440×900 or higher
Operating system: Windows 10 or above & Office Word/Excel 2007 or above.
For furher details, please refer to the user manual, section ‘Installation Requirements’.
Do you have questions regarding the FastGene® qFYR System (FG-QPTC01)? Contact us!
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