FastGene qFYR Real-Time PCR System

Real-time fluorescent quantitative PCR system

  • Fast detection − Probe & dye-based assays
  • Multiplex qPCR − 4 channels
  • Standard plate format − 96-well block with gradient function
  • Highest sensitivity
  • Oustanding homogeneity across all wells of a 96-well plate
  • Software included − Easy-to-use and intuitive
  • Integrated Precision Melt Analysis Tool (for probe-based allelic discrimination)
  • Many applications − Gene expression analysis, genotyping, HRM (high resolution melt), pathogen detection, genetic analysis

PRODUCT INQUIRY

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Cat. No. FG-QPTC01

Details

Introduction

Highest precision instrument

The FastGene® qFYR is a highest precision instrument for performing quantitative polymerase chain reaction (qPCR) experiments. qPCR is a well-established method for the sensitive detection and quantification of nucleic acids. During a measurement, the target DNA or RNA sequence is amplified, while a cycle-dependent increase of a fluorescent signal is detected in real-time.

The device for multiple applications

The FastGene® qFYR was developed to meet highest laboratory standards and deliver reliable performances for various real-time qPCR applications:

  • Gene expression analysis
  • Absolute and relative quantification
  • Endpoint qualitative analysis
  • Genotyping
  • Gene mutation analysis
  • Pathogen detection
  • GMO detection
  • Protein stability screening
  • miRNA analysis
  • Melting curve analysis

Innovative Technology

High sensitivity optical design

The FastGene® qFYR has a unique, patented optical detection system. It combines a high quality PMT (photomultiplier tube) with a Fresnel lens that has a short focal length. The resulting short distance from detector to sample reduces signal loss and cross-talk between samples, and generally improves signal sensitivity.

Superior thermal cycler

The FastGene® qFYR delivers excellent temperature control, temperature precision (± 0.2 °C) and uniformity over the entire 96-well plate. The unique hollowed out thermal block design reduces its overall weight and ensures rapid heating and cooling rates (up to 6 °C per second) for fast qPCR protocols. The system also uses state-of-the-art Peltier components for highest reaction quality and performance stability, providing reliable and consistent qPCR results.

Fast Scanning Mode

4 different fluorescence channels and a double FAM/SYBR channel enabling fast measurements.

Channel 1: FAM/SYBR Green etc.
Channel 2: VIC/JOE/HEX/TET etc.
Channel 3: ROX/Texas Red etc.
Channel 4: Cy5 etc.
Channel 5: FAM/SYBR Green etc.

Powerful User Software

The software has it all

The analysis software (FastGene® qFYR Analysis Studio) impresses with its particularly simple and operation. The menu is clearly structured and intuitively arranged. It responds to the needs of the user for different experimental setups, and personalized settings can be easily adjusted. Integrated analysis algorithms allow many steps, such as baseline subtraction or Cq value threshold calculation, to be performed automatically.
Absolute and relative quantification of nucleic acids can also be automated. The software allows you to save predefined analysis settings for auto-exporting run data into a format of choice, including Excel, PDF, txt export format.

  • Clearly structured and user-friendly
  • Intuitive navigation
  • Customizable settings
  • Automatic analysis with integrated algorithms
  • Modules for multiple applications
  • Modern UI design

Application data

Generate high quality data

The combination of innovative optics and a high-precision thermal block ensure ideal amplification conditions. This makes the FastGene® qFYR ideal for various quantitative real-time PCR applications.

Outstanding homogeneity

FastGene® qFYR shows great homogeneity across all wells of a 96-well plate.

Figure 1: Amplification of 1 ng plasmid DNA in all 96 wells of a PCR plate. The mean Cq value at a cycle number of 13.89 +/- 0.055 was determined automatically, illustrating highly homogenous amplification results obtained by the FastGene® qFYR.

Powerful multiplexing

FastGene® qFYR can discriminate up to five different targets in a single reaction well.

Figure 2: Multiplex amplification of three gene targets (Actb, Gapdh and B2m) that was carried out from 100 ng to 0.1 ng RNA in a OneStep qPCR with the FastGene® Probe OneStep Mix (LS47).

Accurate quantification

The broad dynamic range of the FastGene® qFYR ensures a reliable and accurate quantification.

Figure 3: The amplification plot shows the log of change in normalized reporter fluorescence against the cycle number. The amplification took place from plasmid DNA with AMP specific primers in a 10-fold dilution series ranging from 1 ng to 1×10-6 ng plasmid DNA. The generated standard curve shows 100 % efficiency.

1.3-fold target discrimination

With the FastGene® qFYR, smaller concentration differences can be distinguished with high accuracy, underlining the high sensitivity of the device.

Figure 4: The amplification of plasmid DNA was carried out using AMP-specific primers with a 2-fold dilution series starting at 0.1 ng with additional dilutions of 1:1.3, 1:1.4, 1:1.5 and 1:1.6. Concentration differences can be detected up to 1:1.3-fold dilution.

Melt curve analysis

The Precision Melt Analysis Tool is a powerful method for probe-based allelic discrimination. This function is already included in the FastGene® qFYR analysis software and does not need to purchased additionally.

Figure 5: The difference plot of the high-resolution melting curve allows the discrimination between SNPs extracted from blood samples for brown eyes and two variants of blue eyes.

Gradient qPCR for optimal annealing

The thermal gradient function of the FastGene® qFYR can be used to determine the optimal annealing temperature of a specific target.

Figure 6: Plasmid amplification with IC green dye was carried out in a 10-fold dilution series from 1 ng to 1×10-6 ng. This example of a thermal gradient experiment with 55 °C and 68 °C shows 55 °C to be the optimal annealing temperature with a 100% efficiency.

Specifications

Thermal cycler
Block capacity 96
Sample volume 1–50 µL
Heating and cooling method Peltier (6 temperature control modules)
Temperature control technology Hollow-out module combined with edge temperature compensation technology
The maximum heating and cooling rate of the block 6.0 °C/s
The average heating and cooling rate of the sample 4.0 °C/s
Temperature range 4–100 °C
Temperature accuracy ± 0.2 °C
Temperature uniformity ± 0.2 °C
Temperature setting range 4–100 °C
Heated lid Electronic automatic lid
Gradient zones 12 columns
Temperature gradient temperature range 1–36 °C
Linear dynamic range 10 orders of magnitude: 1-1010 copies
Software name FastGene®qFYR Analysis Studio
Optical detection
Excitation light source 4 single-color high-efficiency LEDs (maintenance-free, working life >100,000 hours)
Detector device Highly sensitive PMT (photo multiplier tube) with Frensel lens
Scanning principle Time-resolved real-time scan
Fluorescent channel number 4 channels (2x FAM)
Application to fluorescent Channel 1: FAM/SYBR Green etc.
Channel 2: VIC/JOE/HEX/TET etc.
Channel 3: ROX/Texas Red etc.
Channel 4: Cy5 etc.
Channel 5: FAM/SYBR Green etc.
Dye compatibility FAM/SYBR Green
VIC/HEX/TET/JOE
ROX/Texas Red, Mustang Purple
Cy5/LIZ
Detector position Top of the block
Detection sensitivity 1 copy of the target sequence
System sensitivity Distinguishable 1.33-fold copy number difference in singleplex reactions
Detection time Standard mode (full channel): 8.5 seconds/96-well plate;
Fast mode (dual FAM): 4 seconds/96-well plate
Exitation/detection range Excitation range: 455–650 nm;
Scope of test: 510–715 nm
Physical specifications
Instrument specifications Dimensions: 35 cm x 52 cm x 37 cm (length x wide x height)
Weight: 25 kg
Environmental conditions Temperature: 10–30 °C
Humidity: ≤85 % RH no condensation
Atmosheric pressure: 85.0 kPa ~ 106.0 kPa
Altitude: < 2000 m
Power supply specification Power supply voltage: AC 100–240 V
Frequency: 50 Hz
Power: 750 W
Data communication interface USB ports

 

Compatibility & Consumables

Get the right consumables for FastGene® qFYR

The FastGene® qFYR is compatible with low-profile (0.1 mL) PCR tubes/8-well PCR tube strips with transparent, flat tops as well as non-skirted or half-skirted low profile 96 well PCR reaction plates (See product category: PCR Plastic (link)):

  • Single tubes: Low-profile (0.1 mL) clear/white PCR thin-walled single tube, flat-topped transparent optical tube cover, e.g.:
  • 8-well strips: Low-profile (0.1 mL) clear/white PCR thin-walled 8-tube, flat-top transparent optical tube cover, e.g.:
    • 8-well PCR Tube Strips 0.1 mL with flat cap strips (FG-017FC)
    • 8-well PCR Tube Strips 0.1 mL with flat caps (FG-018WF)
    • 8-well White PCR Tube Strips 0.1 mL + optical Flat Cap Strips (FG-019FC)
  • 96-well plates: Low-profile (0.1 mL) clear/white PCR thin-walled 96- well plate with no hemline or hemline, e.g.:
    • FastGene® White 96-well PCR Plate 0.1 mL, semi-skirted (FG-2102)
    • FastGene® Two-Component PCR Plate 0.1 mL (with or without barcode) (FG-HD0196BC)
    • FastGene® 96-well PCR Plate 0.1 mL, non-skirted (FG-170350)
    • FastGene® 96-well Fast PCR Plate 0.1 mL (FG-03890-50)

! It is not compatibel with high-profile (0.2 mL) PCR reaction tube and convex tube covers !

Info: If the sample tubes are just a few, use 8-well tubes for support on both sides, or use empty single tubes in the four corners to prevent uneven force on the plate slot.

Dye compatibility

Our FastGene® IC Green and Probe Mixes are the ideal reagents for the FastGene® qFYR.
Find the right dye mix for your application: PCR reagents and enzymes – Quantitative PCR (link).

F1: FAM/SYBR Green
F2: VIC/HEX/TET/JOE, JUN
F3: ROX/Texas Red, Mustang Purple
F4: Cy5/LIZ

Product inquiry

Do you have questions regarding the FastGene® qFYR System (FG-QPTC01)? Contact us!

Downloads

Manual

Manual – FastGene qFYR Real-Time PCR System (FG-QPTC01)

Brochure

Brochure – FastGene qFYR Real-Time PCR System (FG-QPTC01)

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