MIDORIGreen Xtra is a new highly sensitive green fluorescent stain for a safe visualization of DNA and RNA in agarose gels. This DNA stain is a safe and better alternative to the traditional nucleic acid stain ethidium bromide (EtBr). Remarkably, agarose gels stained with MIDORIGreen Xtra have a very low background fluorescence, which makes the identification of low amounts of DNA very easy. Be one of the first and test MIDORIGreen Xtra for a safe detection of DNA with an unbeatable sensitivity. Request your free sample here.
Ultra-high sensitivity of DNA bands detected with MIDORIGreen Xtra. Left: Detection with a Blue/Green LED transilluminator. Right: Detection with a Blue LED transilluminator.
MIDORIGreen Xtra is optimized for Blue/Green and Blue LED light, leading to unbeatable fluorescence signals of DNA and RNA in agarose gels. In addition, UV-light is also suitable for the detection of nucleic acid, but less efficient than non-damaging visible light. Remarkably, MIDORIGreen Xtra did not stain the agarose gel, leading to an excellent signal to noise ratio.
MIDORIGreen Xtra delivers even better DNA/RNA signals than ethidium bromide. However, this innovative DNA stain is non-carcinogenic, non-mutagenic and non-toxic and therefore not harmful for your health. Independent laboratories confirmed its safety: Both the mutagenicity test (ames test) and the cytotoxicity test were negative. Convince yourself and download here our safety report.
MIDORIGreen Xtra is optimized for Blue/Green and Blue LED light, leading to unbeatable fluorescence signals of DNA and RNA in agarose gels. MIDORIGreen Xtra is suitable for In-Gel and Poststaining.
|MIDORI Green DNA dye||Blue/Green LED||Blue LED||UV-Light||In-Gel||Poststaining||Direct|
Compatible, but not recommended
We know that the combination of Midori Green Xtra and Orange G will lead to a white smear while using UV light. To avoid this, please use Blue or Blue/Green LED detection systems or use a different loading dye.
The dye is oppositely charged to the DNA, so if the DNA migrated downwards, the stain will migrate upwards. Therefore, the gel will destain. We recommend to use a higher agarose percentage and a quicker run.
We commend to warm the stain up to RT or 37°C for several minutes. Please vortex the stain before usage.