Qsep Bio-Fragment Analyzer

Capillary gel electrophoresis for DNA, RNA and proteins

  • Fully automated fragment analyzer for DNA, RNA and protein analysis
  • Easiest handling thanks to ready-to-use, exchangeable cartridges with integrated capillaries, fluorophores and gel matrices (patented technology (link))
  • Perfect results and reproducible results without extensive training
  • 4 models are available differing in number of samples that can be performed per run:
    • Qsep1 series:
      • Qsep1: For 1 to 8 samples (1 channel)
      • Qsep1-Plus: For 1 to 16 samples (1 channel)
    • Qsep100: For up to 96 samples (1 channel)
    • Qsep400: For up to 96 samples (4 channels)

PRODUCT INQUIRY

Cat. No. C400100, C100100, C100001, C100001-P 1 piece
Qsep Fragment Analyzer with inserted cartridgeinside

Details

Technology

The Qsep Bio-Fragment Analyzer replaces old-fashioned, labor-intensive gel analysis of DNA, RNA and proteins. The single-channel capillary electrophoresis (CGE) system automates sensitive, high-resolution analysis of 1 to 96 samples, in 2-7 minutes per run. Ready-to-run gel cartridges allow up to 200 samples to be analyzed with a minimum of hands-on interaction, reducing manual handling errors and eliminating the need for gel preparation. An intuitive Q-Analyzer software ensures convenient analysis and documentation of data.

The Qsep Bioanalyzers provide accurate results in three simple steps:

  1. Insert the disposable pen-shaped gel cartridge into the instrument.
  2. Place the samples in the sample tray.
  3. Choose the appropriate method and run. In just a few minutes, users can get reliable results in diverse formats (PDF, Word, Excel), including peak and gel charts.

Qsep performance in three steps

Qsep instruments

The four Qsep Fragment Analyzer models differ in the number of samples that can be performed per run.
Handling, technology as well as the type and quality of the feasible applications are identical.

 

Qsep1: For 1 to 8 samples (1 channel)

The Qsep1 instrument is comprised of a capillary gel electrophoresis (CGE) core integrated with a high sensitivity LED-induced fluorescence (LEDIF) detector and 1 to 8 well auto-sampler. The replaceable gel-cartridge automatically regenerates the capillary gel between runs and can be used for up to 200 sample injections providing robust and superior results for all kinds of molecular genetic analysis applications.

Qsep1 with variety of replaceable gel-cartridge kit is specifically designed for DNA, RNA and Protein analysis providing reproducible and accurate test results in matter of seconds.

Qsep1 comes with Q-Analyzer software which has a simple-to-use User Interface (UI) that delivers consistent and high-precision data analysis and data export functions helping users to turn molecular biology into “Moving” biology.

 

Qsep1-Plus: For 1 to 16 samples (1 channel)

Qsep1-Plus is ideal for small laboratories with low throughput (1~15 samples) needs. If only profiling is needed, the sample capacity can be up to 16 samples.

 

 

Qsep 100: For up to 96 samples (1 channel)

Qsep100 is a small, simple and user friendly bench top capillary gel electrophoresis (CGE) system integrated with a high sensitivity LED-induced fluorescence (LEDIF) detector with an easily-replaceable gel cartridge and 96-well auto-sampler. The 150 mm long capillary mounted into the cartridge contains a gel matrix that is replenished from the top reservoir of the cartridge automatically between sample runs. The reservoir provides sufficient gel for up to 200 sample injections providing robust and superior results for all kinds of molecular genetic analysis applications.

Qsep100 with variety of replaceable gel-cartridge kit is specifically designed for DNA, RNA and Protein analysis providing reproducible and accurate test results in matter of seconds.

Qsep100 comes with Q-Analyzer software which has a simple-to-use User Interface (UI) that delivers consistent and high-precision data analysis and data export functions helping users in their daily molecular biology experiments.

 

Qsep 400: For up to 96 samples (4 channels)

Qsep400 has the highest throughput in the Qsep family. The 4-channel instrument is designed as a 4-capillaries gel electrophoresis (CGE) system, integrated with a high sensitivity LED-induced fluorescence (LEDIF) detector with an easily-replaceable 4-channel gel-cartridge and 96-well auto-sampler, which can analyze / detect four samples at the same time.

Qsep400 has been designed for the larger research and clinical laboratories, who have large sample volumes. The high-throughput system provides high-speed analysis, superior results for all kinds of molecular genetic analysis applications. Qsep400 with variety of replaceable gel-cartridge kit is specifically designed for DNA, RNA and Protein analysis providing reproducible and accurate test results in matter of seconds.

Qsep400 comes with Q-Analyzer software which has a simple-to-use User Interface (UI) that delivers consistent and high-precision data analysis and data export functions helping users in their daily molecular biology experiments.

Availability

The Qsep Fragment Analyzers are available in Germany, Austria, Belgium, Denmark, Finnland, Greece, Croatia and Serbia. For other countries, please contact us:

Specifications

Specifications
Qsep1
Qsep1-Plus
Qsep100
Qsep 400
Qsep1 Bio-Fragment Analyzer | Front view
Qsep1-Plus Bio-Fragment Analyzer | Front view
Qsep100 Bio-Fragment Analyzer | Front view
Qsep400 Bio-Fragment Analyzer | Front view
Cat. No. C100001 C100001-P C100100 C400100
LED Light Source 525 nm 525 nm 525 nm 525 nm
Fluorescence Detection 590 nm 590 nm 590 nm 590 nm
Separation Voltage 1-8 KV 1-8 KV 1-15 KV 1-15 KV
Connection WiFi and Cable WiFi and Cable USB USB
Pour Source AC 100-240V AC 100-240V AC 100-240V AC 100-240V
Maximum Power 30W 30W 30W 60W
Dimension 240 mm x 210 mm x 300 mm 240 mm x 210 mm x 300 mm 380 mm x 300 mm x 400mm 540 mm x 400 mm x 360mm
Weight 5.5 kg 5.5 kg 15 kg 26 kg
Highlights
System Type 1 Channel Portable System 1 Channel Portable System 1 Channel System 4 Channel System
Automated Sampling 1-8 samples 1-16 samples 1-96 samples 1-96 samples
Rapid Analysis 2-7 min/sample 2-7 min/sample 2-7 min/sample 2-7 min/ 4 samples
Resolution 1-4 bp (between 100-500 bp) 1-4 bp (between 100-500 bp) 1-4 bp (between 100-500 bp) 1-4 bp (between 100-500 bp)
Detection Sensitivity 5 pg/uL (2 pg/µL) 5 pg/uL (2 pg/µL) 5 pg/uL (2 pg/µL) 5 pg/uL (2 pg/µL)
Minimum Sample Volume 2 µL (Micro-Vial) 2 µL (Micro-Vial) 2 µL (Micro-Vial) 2 µL (Micro-Vial)
Software Q-Analyzer Q-Analyzer Q-Analyzer Q-Analyzer

L.O.D.: 2pg/ul (if diluted in water)

Applications

Standard Cartridge (S2) High Resolution Cartridge (S1) Kilobase Cartridge (S3) Fast Cartridge (F3)
Only suitable for Qsep100		 High Sensitivity Cartridge (N1) RNA Cartridge (R1) Protein Cartridge (P2) High sensitivity Kilobase Cartridge (N3) High sensitivity RNA Cartridge (NR1)
PCR product screening x x x
Restriction Fragment Length Polymorphism (RFLP) x x x
Plasmid purification & Vector cloning analysis x x
Next Generation Sequencing (NGS) QC x
Genomic DNA analysis x x
Cell free DNA detection x
RNA Analysis x x
Low concentration sample x x x
CRISPR QC & Analysis x x x x
Large size analysis (>50kb) x x
Replace SDS-PAGE x
IgG Purity Test x
Cartridges

DNA

Standard Cartridge (S2) High Resolution Cartridge (S1) Fast Cartridge (F3)
Only suitable for Qsep100
 High Sensitivity Cartridge (N1) Kilo Base Cartridge (S3) High Sensitivity Kilo Base Cartridge (N3) Quantitative Standard Cartridge (S2) Quantitative High Resolution Cartridge (S1)
Cat. No. C105201 (2 pcs) C105202 (2 pcs) C105203 (2 pcs) C105105 (1 pc) C105206 (2 pcs) C105153 (1 pc) C105201-Q (2 pcs) C105202-Q (2 pcs)
C105801 (8 pcs) C105802 (8 pcs) C105803 (8 pcs) C105205 (2 pcs) C105806 (8 pcs) C105253 (2 pcs)
C405101 (1 pc) C405102 (1 pc) C405103 (1 pc) C405105 (1 pc) C405106 (1 pc) C405101-Q (1 pc) C405102-Q (1 pc)
Sample size range 10-5000 bp 10-5000 bp 10-5000 bp 10-5000 bp 10-50000 bp 10-50000 bp 10-1500 bp 10-1500 bp
L.O.D. 0.1 ng/µL* 0.1 ng/µL* 0.1 ng/µL* 5 pg/µL* 0.1 ng/µL* 5 pg/µL* 0.1 ng/µL* 0.1 ng/µL*
Best Resolution 4-10 bp 1-4 bp >50 bp >10 bp 10-50 bp 10-50 bp 4-10 bp 1-4 bp
Analysis time (per sample) 2-3 mins 3-5 mins 1-2 mins 2-3 mins 5-21 mins 9-25 mins 2-3 mins 3-5 mins
Sample number (per cartridge) 200 runs 200 runs 300 runs 100 runs 200 runs 100 runs 200 runs 200 runs
Sample consumption (per run) <0.1 µL <0.1 µL <0.1 µL <0.1 µL <0.1 µL <0.1 µL <0.1 µL <0.1 µL
Recommended sample volume 0.1 mL tube: 10 µL 0.1 mL tube: 10 µL 0.1 mL tube: 10 µL 0.1 mL tube: 10 µL 0.1 mL tube: 10 µL 0.1 mL tube: 10 µL 0.1 mL tube: 10 µL 0.1 mL tube: 110 µL
0.2 mL tube: 20 µL 0.2 mL tube:
20 µL		 0.2 mL tube:
20 µL		 0.2 mL tube:
20 µL		 0.2 mL tube:
20 µL		 0.2 mL tube:
20 µL		 0.2 mL tube:
20 µL		 0.2 mL tube:
20 µL
Micro vial:
2 µL (C104250)		 Micro vial:
2 µL (C104250)		 Micro vial:
2 µL (C104250)		 Micro vial:
2 µL (C104250)		 Micro vial:
2 µL (C104250)
Shelf life 6 months 6 months 4 months 3 months 6 months 3 months 6 months 6 months

* Determined by the DNA ladder 15-622 (DNA size marker C109200)
L.O.D.: 2pg/µL (if diluted in water)

RNA and Proteins

RNA Cartridge (R1) High Sensitivity RNA Cartridge (NR1) Protein Cartridge (SDS) (P2)
Cat. No. C105110 (1 pc) C105111 (1 pc) C105121 (1 pc)
C105210 (2 pcs) C105211 (2 pcs) C105221 (2 pcs)
C105810 (8 pcs)
C405110 (1 pc) C405121 (1 pc)
Sample size range N/A N/A 14-150 kDa
L.O.D. 5 ng/µL 1 ng/µL 0.5 ng/µL (BSA)
Best Resolution N/A N/A N/A
Analysis time (per sample) 5-10 mins 5-10 mins 6 mins (BSA)
10 mins (IgG)
Sample number (per cartridge) 100 runs 100 runs 100 runs
Sample consumption (per run) <0.1 µL <0.1 µL <0.1 µL
Recommended sample volume 0.1 mL tube: 10 µL 0.1 mL tube: 10 µL 0.1 mL tube: 10 µL
0.2 mL tube: 20 µL 0.2 mL tube: 20 µL 0.2 mL tube: 20 µL
Micro vial: 1 µL Micro vial: 1 µL
(C104250) (C104250)
Shelf life 3 months 3 months 3 months

L.O.D.: 2pg/µL (if diluted in water)

Downloads

Manuals

F0045_Qsep Series Operation Manaual-Software-ENG-A
F0042-Qsep1 Quick start installation CHT&ENG
F0016-Qsep1-Quick start operation-ENG-C-20190903
F0002-Software Installation Quick Start -ENG-E
F0006-Q-Viewer and Q-Editor Quick Start -ENG-D

Flyer

Flyer Qsep Fragment Analyzer
Flyer Qsep1 Biofragment Analyzer
Flyer Qsep100 Biofragment Analyzer

Patent

Patent BiOptic European Patent Specification

Publications

Qsep Bio-Fragment Analyzer: NGS Library Validation Using Automated Capillary Gel Electrophoresis

Product Videos

NEW video: Qsep Bio-Fragment Analyzer Series

Operating procedure | Different devices for different requirements

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Qsep 100 Bio-Fragment Analyzer

Applications | Cartridges | Handling and procedure

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Product Inquiry

Do you have questions regarding the product?

Availability: The Qsep Fragment Analyzers are available in Germany, Austria, Belgium, Denmark, Finnland, Greece, Croatia and Serbia. For other countries, please contact us:

1 review for Qsep Bio-Fragment Analyzer

  1. Gotsmann, Eukaryontengenetik, Technische Universität Kaiserslautern –

    Das Gerät ist absolut Spitze! Auflösung und Sensitivität von dem Gerät sind wirklich toll. Wir haben den Qsep mittlerweile fest in unsere workflows für RNA-Seq und Ribo-Seq Experimente integriert und ich habe bereits einige NGS libraries damit analysiert. Bisher lief immer alles problemlos und wir sind froh, dass wir unsere libraries endlich auch in-house analysieren können, bevor wir sie zum Sequenzieren verschicken. Vor allem die Messung in den micro-vials ist sehr bequem, gerade wenn die Ausbeute bei der library prep eher gering war.

    Translation by NIPPON Genetics EUROPE:
    The device is absolutely great! The resolution and sensitivity of the device are really great. We have now firmly integrated the Qsep into our workflows for RNA-Seq and Ribo-Seq experiments and I have already used it to analyze several NGS libraries. So far, everything has always run smoothly and we are happy that we can finally analyze our libraries in-house before we send them off for sequencing. Above all, the measurement in the micro-vials is very convenient, especially when the yield from library prep was rather low.

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