qPCR Workflow
Your complete qPCR workflow
NIPPON Genetics EUROPE provides everything you need for an efficient and reproducible qPCR workflow, from sample preparation to reliable results with a FastGene® qFYR Real-Time PCR System.
Prepare your sample
Cell freezing (Bambanker™ Cell Freezing Medium)
RNA purification (FastGene® RNA Kits)
Reverse transcription (FastGene® Scriptases)
DNA purification (FastGene® gDNA Kit)
Amplify & analyse
Amplification (PCR Biosystmes qPCR Mixes, Plastic Consumables)
Analysis (FastGene® qFYR Real-Time PCR Systems)
Prepare your sample
Every successful qPCR workflow starts with sample preparation. With solutions for cell preservation, RNA isolation, reverse transcription, and genomic DNA purification, NIPPON Genetics Europe supports your sample workflow from the very first step, helping you build a reliable foundation for accurate qPCR results.
Bambanker™ Cell Freezing Medium
Ready-to-use cell freezing medium designed to support reliable cell preservation and recovery of valuable cell samples.
Product |
Description |
Cat. No. |
| Bambanker (20 mL) | For all cell lines | BB03 |
| Bambanker (100 mL) | For all cell lines | BB05 |
| Bambanker Direct (20 mL) | For hybridoma cells | BBD01 |
| Bambanker HRM (20 mL) | For ES and iPS cells | BBH01 |
| Bambanker DMSO-free (20 mL) | For most sensitive cells | BBF01 |
FastGene® RNA Kits
Choose the RNA isolation kit that fits your workflow: from fast and efficient RNA purification with the Basic Kit to complete DNA removal and concentrated elution with the Premium Kit.
Product |
Description |
Cat. No. |
| FastGene® RNA Basic Kit (50 preps) | Reliable and fast purification | FG-80050 |
| FastGene® RNA Basic Kit (250 preps) | Reliable and fast purification | FG-80250 |
| FastGene® RNA Premium Kit (50 preps) | Ultrapure & concentrated RNA, free of genomic DNA | FG-81050 |
| FastGene® RNA Premium Kit (250 preps) | Ultrapure & concentrated RNA, free of genomic DNA | FG-81250 |
FastGene® Scriptases
FastGene® Scriptase enzymes provide the right solution for different RNA inputs, from basic templates to low-input samples, long cDNA fragments, and complex RNA secondary structures.
Product |
Cat. No. |
| FastGene® Scriptase Basic (20000 U) | LS52 |
| FastGene® Scriptase II (20000 U) | LS53 |
| FastGene® Scriptase III (20000 U) | LS55 |
| FastGene® Scriptase Basic cDNA Kit (100 rxns) | LS62 |
| FastGene® Scriptase II cDNA 5x ReadyMix (100 rxns; random hexamers) | LS63 |
| FastGene® Scriptase II cDNA 5x ReadyMix (100 rxns; Oligo-dTs, random hexamers) | LS64 |
| FastGene® Scriptase III cDNA Kit (100 rxns) | LS66 |
| FastGene® Scriptase III cDNA 5x ReadyMix (100 rxns; random hexamers) |
LS67 |
FastGene® gDNA Kit
Extract high-quality genomic DNA from blood, tissue, or cultured cells with an easy protocol, ready for downstream applications such as PCR and qPCR.
Product |
Cat. No. |
| FastGene® Blood & Tissue gDNA Extraction Kit (50 preps) | FG-70050 |
| FastGene® Blood & Tissue gDNA Extraction Kit (250 preps) | FG-70250 |
Amplify and Analyse
Once your sample is prepared, every detail matters. With optimized qPCR mixes, reliable plastic consumables, and an intuitive FastGene® qFYR System, you can build a complete setup for accurate detection, amplification, and efficient qPCR analysis.
PCR Biosystem qPCR Mixes
PCRBIO qPCR mixes provide ideal solutions for different qPCR assay formats, supporting sensitive detection, reproducible amplification, and efficient real-time PCR workflows.
Product |
Cat. No. |
| qPCRBIO SyGreen Mix Separate-ROX (5 x 1 mL) | PB20.14-05 |
| qPCRBIO SyGreen Mix Separate-ROX (20 x 1 mL) | PB20.14-20 |
| qPCRBIO SyGreen Mix Separate-ROX (50 x 1 mL) | PB20.14-51 |
| qPCRBIO Probe Blue Mix Separate-ROX (5 x 1 mL) | PB20.27-05 |
| qPCRBIO Probe Blue Mix Separate-ROX (20 x 1 mL) | PB20.27-20 |
| qPCRBIO Probe Blue Mix Separate-ROX (50 x 1 mL) | PB20.27-50 |
| Clara™ Probe Mix Separate-ROX (1 mL) | PB20.64.01 |
| Clara™ Probe Mix Separate-ROX (3 x 1 mL) | PB20.64.03 |
| Clara™ Probe Mix Separate-ROX (5 x 1 mL) | PB20.64.05 |
Plastic Consumables
Reliable qPCR plastics support precise reaction setup, efficient sealing, minimal evaporation, and consistent optical detection for reproducible qPCR results.
Product |
Cat. No. |
| White 8-well PCR Tube Strips 0.1 mL with Flat Cap Strips (125) | FG-019FC |
| White 96-well PCR Plate 0.1 mL, semi-skirted (50) | FG-210250 |
| FastGene® White 384-well PCR Plate 40 μL, full-skirted (50) | FG-310050 |
| FastGene® Adhesive PCR Foil (100) | FG-93AC2 |
FastGene® qFYR Real-Time PCR Systems
Complete your qPCR workflow with FastGene® qFYR systems in 96 well or 384 well format, including 4+1 channel and 6 channel Plus variants for flexible and scalable quantification.
Product |
Cat. No. |
| FastGene® qFYR (96-well Real-Time PCR System, 4 + 1 channels) | FG-QPTC01 |
| FastGene® qFYR Plus (96-well Real-Time PCR System, 6 channels) | FG-QPTC02 |
| FastGene® qFYR 384 (384-well Real-Time PCR System, 4 + 1 channels) | FG-QPTC03 |
| FastGene® qFYR 384 Plus (384-well Real-Time PCR System, 6 channels) | FG-QPTC04 |